Nematode suppression with brassicaceous amendments: application based upon glucosinolate profiles

Glucosinolate profiles differ among plant species and their isothiocyanate (ITC) derivatives differ in toxicity to nematodes. Successful management of plant–parasitic nematodes by ITCs requires the incorporation of appropriate amounts of glucosinolate-containing biomass. Plant materials, containing glucosinolate-precursors of the ITCs most toxic to nematodes, were selected and applied to soil based upon ITC lethal concentration (LC) values. This provided a reliable and repeatable basis for application rates for suppression of Meloidogyne javanica and Tylenchulus semipenetrans by Brassica hirta and M. javanica by B. juncea. Sufficient biomass of B. hirta to potentially yield 0.03–0.12 mmol ml of glucotropeolin reduced nematode survival compared to similar amounts of broccoli (Brassica oleraceae var. botrytis). At biomass levels providing .0.37 mmol ml of glucotropeolin, mortality of M. javanica was 100% with B. hirta. Biomass of B. juncea potentially yielding 2.82 mmol ml of sinigrin reduced M. javanica survival 65% below that obtained by a similar amount of broccoli. Rates of B. juncea to yield lethal levels of allyl ITC to reduce T. semipenetrans survival underestimated the glucosinolate application rates for this amendment. Application of plant biomass to soil .2.9% w/w reduced M. javanica survival regardless of the glucosinolate concentration of the amendment material. Application of brassicaceous amendments to soil initiates complex and dynamic biological and chemical processes. Despite the inherent complexity, we find that brassicaceous amendments can be applied to achieve consistent and repeatable nematode suppression when based upon the chemistry of the incorporated material. q 2004 Published by Elsevier Ltd.